Protocols

Animal Protocols

FDB Plasmid Electroporation

Mouse Grip Strength

Hindlimb Unloading

Leucine Administration

Glucose Tolerance Test

Insulin Tolerance Test

TA Plasmid Electroporation

Transgenic Genotyping

Bupivacaine Injection

Deuterium Administration

Mouse Electrophysiology

Histology

SDH Staining

Sirius Red Staining

H&E Staining

Cryosectioning

Fiber Type Analysis by IF

Tissue Freezing in OCT

Mitochondrial Physiology

Muscle Fiber Bundle Separation and Permeabilization

Mitochondrial Respiration

Mitochondrial H2O2 Emission

Molecular Biology

DNA Maxiprep

DNA Miniprep

Bacterial media and antibiotic preps

Immunoblotting

Muscle Tissue Powdering

Real Time PCR

Muscle Protein Synthesis by Deuterium Labeling

Tissue Culture

Myotube Differentiation

C2C12 Growth and Differentiation Media Recipes

Lipofectamine mediated Plasmid Transfection